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. 2017 Jun 21;8:1026. doi: 10.3389/fpls.2017.01026

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Copyright © 2017 Kytidou, Beenakker, Westerhof, Hokke, Moolenaar, Goosen, Mirzaian, Ferraz, de Geus, Kallemeijn, Overkleeft, Boot, Schots, Bosch and Aerts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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N-glycans of α-galactosidases. (A) SDS-PAGE analysis followed by Coomassie Brilliant Blue staining of 1 μg pure plant produced proteins and 3 μg of Fabrazyme. E = protein treated with Endo H, F = protein treated with PNGase F, C = untreated protein. (B–D) N-glycosylation profiles were determined by MALDI-TOF MS analysis of 2-aminobenzoic acid derivatised PNGase-A released N-glycans of plant produced galactosidases. Glycan structures depicted were deduced from the measured m/z values. Blue square, N-acetylglucosamine; red triangle, fucose; open star, xylose; green circle, mannose. Presented N-glycan profiles of the following proteins: (B) α-GAL, (C) α-NAGAL, (D) α-NAGAL^EL.