Table 1.

Overview of enzyme purifications.

Enzymes	Purification steps	Volume (ml)	Total protein (mg)	Total activity (μmol/h)	Total specific activity (μmol/h/mg)	Purification fold	% Yield = recovery of activity
α-GAL	Plant extract	49	136	3412	25	0	100
	Con-A	3	1.3	1928	1444	57	57
	Cation exchange	2	0.4	860	2151	86	25
	Gel filtration	1.4	0.12	308	2585	103	9
α-NAGAL	Plant extract	38	135	319	2.3	0	100
	Con-A	3	1	112	107	45	35
	Anion exchange	4	0.7	94	132	56	30
	Gel filtration	2	0.14	29.5	211	89	9
α-NAGALEL	Plant extract	25	64	314	5	0	100
	Con-A	2	0.45	122	270	55	49
	Anion exchange	4	0.2	55	273	56	17
	Gel filtration	1.2	0.056	28	501	102	9