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. 2017 Jun 21;8:1159. doi: 10.3389/fmicb.2017.01159

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Copyright © 2017 Yamashita, Matsumoto, Endo, Ukibe, Hosoya, Matsubara, Nakagawa, Sakai and Miyazaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of intraperitoneal inoculation of L. helveticus SBT2171 on the number of immune cells in the lymph nodes. Inguinal lymph nodes were collected at day 42 from CIA mice given L. helveticus SBT2171 or PBS (control) intraperitoneally. Immune cells were isolated from the lymph nodes and the numbers of total immune cells (A), B cells (B), CD4⁺ T cells (C), and CD8⁺ T cells (D) were analyzed by flow cytometry. Data are presented as box plots (n = 8). ^∗P < 0.05, compared to the control group by the Mann–Whitney U-test.