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. 2017 Jun 21;8:908. doi: 10.3389/fpls.2017.00908

FIGURE 4.

FIGURE 4

Effects of silencing OsBSK1-2 on flg22- and chitin-triggered immune responses. Leaf strips of 4-week-old plants were treated with 1 μM flg22 peptide (Pacific immunology) or 20 ug/ml chitin (Sigma) for 12 h. The mock treatment was ddH2O. The expression levels of two defense marker genes -Os04g10010 (A) and -OsPR10b (B) were measured by qRT-PCR. Gene expression levels for the target gene were normalized to the ubiquitin gene. Data shown were normalized to the Kitaake mock-treated (12 h) samples (100%). The Results from two independent biological experiments were combined with statistical analysis. The letters indicate significant differences (one-way ANOVA followed by post hoc Tukey HSD analysis).