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. 2017 Jun 21;8:1179. doi: 10.3389/fmicb.2017.01179

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Copyright © 2017 Liu, Wang, Gao, Han and Xiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results of PCR with primers DCF4/DCR4 with DNA isolated from decoction and adulterants. Lane 1, pure O. sinensis DNA (~120 ng); lanes 2, 3, and 4, amplification products from DNA (~30 ng) isolated from O. sinensis decoction boiled for 60 min; lanes 5, 6, and 7, amplification products from DNA (~30 ng) isolated from decoction boiled for 90 min; lanes 8, 9, 10, 11, and 12, results of PCR with pure DNA (~30 ng) isolated from adulterants C. liangshanensis, C. militaris, O. nutans, C. gunnii, and C. cicadae, respectively.