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. 2005 Jan 21;102(5):1484–1489. doi: 10.1073/pnas.0409168102

Fig. 3.

Fig. 3.

Effect of 3βAdiol on cell proliferation and AR in VP. (A) The effect of 3βAdiol on proliferation in the neonatal VP. Two-week-old WT mice were treated with 3βAdiol and killed 2 h after s.c. injection of BrdUrd (100 mg/kg). Dissected VP was fixed and sectioned for immunostaining with anti-BrdUrd antibody. The percentage of BrdUrd-positive cells in the epithelium was 2.32 ± 0.65% in WT mice and was increased 2-fold to 4.43 ± 1.34% after treatment with 3βAdiol. (B) Immunohistochemical staining for AR in 2-week-old ERα-/-, CYP7B1-/-, and WT mice. Intensity of AR staining in the epithelial cells of CYP7B1-/- mice was higher than it was in WT mice. Antibodies used were anti-AR N-20 (1:300 dilution).