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. 2005 Jan 24;102(5):1584–1589. doi: 10.1073/pnas.0409191102

Fig. 2.

Fig. 2.

Radiation sensitivity and chromosomal instability of nibrin-deficient B lymphocytes. (A) Radiation sensitivity of Nbnlox-6/Δ6 and NbnWT/Δ6 B lymphocytes after deletion of exon 6 of Nbn by tat-Cre in vitro. Cells were labeled with CFSE and activated with LPS/IL4 for 3 days. Cells were then not irradiated intentionally (black line), or γ-irradiated with 1.5 Gy (blue) or 3.6 Gy (green line). Two days later, proliferation and survival of the cells was analyzed cytometrically. Proliferative history of surviving cells was determined according to loss of CFSE label and numbers of surviving cells relative to reference beads (TrueCount, Becton Dickinson), standardizing the numbers of recorded viable cells not stained for propidium iodide. (B) Chromosomal instability of LPS/IL4-activated Nbnlox-6/Δ6 B lymphocytes after treatment with tat-Cre in vitro. On day 3 of culture, metaphase spreads were prepared from cells 3 h after γ-irradiation (1 Gy). Metaphases were Giemsa-stained and scored for aberrant chromosome numbers and apparent chromosomal breaks (-Cre/-Gy, 102 metaphases analyzed; -Cre/+Gy, 59 metaphases; +Cre/-Gy, 236 metaphases; +Cre/+Gy, 138 metaphases).