Table 2. Induction of switch transcripts.
Culture | Mouse Id. | Genotype | Iμ–Cμ transcripts versus GAPDH transcripts* | Iγ1–Cγ1 transcripts versus GAPDH transcripts* | Percent IgG1 cells† |
---|---|---|---|---|---|
43 | CD19-Cre NbnWT/lox-6 | 24.3 | 4.1 | 9.4 | |
I | 44 | CD19-Cre Nbnlox-6/ins6 | 20.7 | 4.1 | 3.2 |
42 | CD19-Cre Nbnlox-6/lox-6 | 21.8 | 4.3 | 4.3 | |
31 | CD19-Cre NbnWT/ins6 | 14.6 | 2.4 | 27.3 | |
II | 35 | CD19-Cre Nbnlox-6/ins6 | 19.4 | 7.2 | 7.9 |
33 | CD19-Cre Nbnlox-6/lox-6 | 16.7 | 4.3 | 4.2 | |
51 | CD19-Cre NbnWT/lox-6 | 23.3 | 10.0 | 4.8 | |
III | 52 | CD19-Cre Nbnlox-6/ins6 | 21.9 | 8.4 | 2.5 |
46 | CD19-Cre Nbnlox-6/lox-6 | 22.1 | 7.5 | 1.8 |
Id., identification.
RT-PCR analysis was performed on day 2 after LPS/IL-4 activation
IgG1 analysis was performed on day 3 after LPS/IL-4 activation