Figure 6.

Knockdown of IRS1/2 suppresses chronic myelogenous leukemia (CML) cell proliferation and glucose metabolism

(A) Protein levels of IRS1 and IRS2 as determined by western blot in K562 cells transfected with siRNAs targeting IRS1 and IRS2, respectively. (B) MTT assay performed in K562 cells transfected with siRNAs targeting IRS1 or IRS2 at 0, 24, 48, and 72 hr after transfection. (C) Percentage of cell death in K562 cells transfected with siRNAs targeting IRS1 or IRS2. Cells were cultured in medium without serum. (D) Levels of glucose uptake in K562 cells transfected with siRNAs targeting IRS1 or IRS2. All assays were performed in three independent experiments, mean ± SEM, *P<0.05