Fig. 2.

Generation and characterization of transgenic mice overexpressing Angptl4 in cardiomyocytes. (A) Diagram of construct used to generate MHC-Angptl4 mice. The lengths of each segment in the transgene are indicated under the construct. NotI denotes the restriction enzyme used to liberate the transgene before injection into fertilized eggs. (B) Determination of levels of transgenic Angptl4 expression in different lines of MHC-Angptl4 mice by Northern blotting analysis. Ctrl, nontransgenic littermates. (C) Detection of transgenic expression of Angptl4 protein in the heart by Western blotting for c-myc tag. Minus and plus signs denote the origin of tissues from nontransgenic (minus sign) or transgenic (plus sign) mouse. Tg, transgenic.