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. 2005 Jan 19;102(5):1767–1772. doi: 10.1073/pnas.0409564102

Fig. 3.

Fig. 3.

Dosage-dependent inhibition of cardiac TG content (A) and LPL activity (B) by transgenic expression of Angptl4, tissue-associated LPL activity (C) and heparin-releasable LPL activity (D) in control and MHC-Angptl4 mice. Hearts from 9-month-old MHC-Angptl4 mice or control littermates were rapidly removed, and ventricles were dissected. Cardiac TG accumulation was assayed as described in Materials and Methods. LPL activity in heart, liver, skeletal muscle, and adipose tissue (WAT) of MHC-Angptl4 mice or control littermates were measured by using [3H]triolein as substrate. LPL activity was significantly decreased only in the hearts of MHC-Angptl4 mice (C). (D) Heparin-releasable LPL activity was determined in 2-month-old MHC-Angptl4 mice or control littermates. Plasma was collected 5 min after heparin injection, and LPL activity was determined as in C. Heparin-releasable LPL activity was similar between MHC-Angptl4 and control mice before (pre-heparin) and after (post-heparin) heparin injection. n = 4 – 6 mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.0001.