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. 2017 Jun 7;2017:6051874. doi: 10.1155/2017/6051874

Figure 5.

Figure 5

miR-Let7A attenuates the expression of cleaved PARP, a cell death marker in bEnd.3 cells under high glucose in vitro condition. To confirm the effect of miR-Let7A on the expression of cleaved PARP in high glucose-treated bEnd.3 cells, immunostaining was performed. Cells in three randomly selected fields were measured for immunodensity using ImageJ software (ImageJ, Madison, Wisconsin, USA). ∗p < 0.05 and ∗∗p < 0.001 compared with nontreated control cells; ϕp < 0.05 compared with Let7A mimic overexpressed control cells; †p < 0.05 compared with Let7A inhibitor overexpressed control cells; ‡p < 0.05 compared with 25 mM glucose-treated cells; ƒp < 0.05 compared with 25 mM glucose-treated and Let7A-overexpressed cells. Scale bar: 50 μm, 4′,6-diamidino-2-phenylindole (DAPI): blue, cleaved PARP: green, Glu: D-glucose treatment for 24 hours, Let7A mimic: Let7A mimic pretreatment for 48 hrs, Let7A inhibitor: anti-Let7A pretreatment for 48 hrs, and PARP: poly ADP-ribose polymerase.