TABLE 1 .
Selection by serial passage of SCVs for faster growth
| Strain | Genotypea | No. of independent evolved lineages | No. of reversion or intragenic mutations | No. of lineages with extragenic mutations | Class of genes with extragenic mutations (n) identified by WGSb |
|---|---|---|---|---|---|
| AH671 | hemC E70fs | 10 | 10 | ||
| AH646 | hemC D74Y | 12 | 12 | ||
| AH727 | hemC D104Y | 22 | 22 | ||
| AH868 | hemH K158UAA | 20 | 9 | 11 | tRNA Ser (4), tRNA Tyr (5), ribosomal protein rpsE (1) |
| AH847 | menA W15UGA | 10 | 7 | 3 | Release factor 2 prfB (3) |
| AH855 | menB D98G | 10 | 10 | ||
| AH875 | menB D151N | 34 | 26 | 8 | tRNA Asp (2), srrA (1), srrB (5) |
| AH635 | menE Y31UAA | 22 | 6 | 16 | tRNA Gln (5),c tRNA Glu (1), tRNA Leu (1), tRNA Lys (4),c tRNA Ser (4), tRNA Tyr (3) |
a
The hem or men mutations indicated are the full genotype of each SCV, with the exception of AH847 (menA W15UGA SAOUHSC-00698 L319S) and AH855 (menB D98G fusA W120L SAOUHSC-02417 A114S).
b
Some of the evolved strains had acquired 1 to 3 additional extragenic mutations (see Table S2 for details). The number in parentheses (n) indicates the number of independent isolates of mutations affecting each gene class.
c
Two of the evolved strains had each acquired mutations affecting two different tRNA genes (see Table S2 for details).