TABLE 4 .

Mutations in menB, srrA, and srrB increase mRNA level of genes in fermentation pathways for ATP production

Gene no., name	Gene function	Gene expression (log2 ratio)
		Men			Hem (SCV vs WT)d
		SCV vs WTa	srrA vs SCVb	srrB vs SCVc
Pyruvate fermentation
SAOUHSC_00113, adhE	Alcohol dehydrogenase	2.1	3.2	3.3	1.6
SAOUHSC_00608, adh1	Alcohol dehydrogenase I	3.1	3.3	3.6	3.5
SAOUHSC_00206, ldh1	l-Lactate dehydrogenase	6.8	−0.2	−0.3	3.4
SAOUHSC_02922, ldh2	l-Lactate dehydrogenase	−0.6	2.9	2.8	0.8
SAOUHSC_00188, pflA	Pyruvate formate-lyase 1 activating enzyme	1.4	6.5	6.6	6.1
SAOUHSC_00187, pflB	Formate acetyltransferase	1.5	6.5	6.5	6.2
Amino acid fermentation
SAOUHSC_01451, ilvA1	Threonine dehydratase	2.4	5.7	6.2	5.8
SAOUHSC_01452, ald1	Alanine dehydrogenase	2.8	5.7	6.1	6.1
SAOUHSC_02839	l-Serine dehydratase	0.5	2	1.2	0.4
SAOUHSC_02840	l-Serine dehydratase	0.4	2.2	1.5	0.5
Arginine deiminase pathway
SAOUHSC_02969, arcA	Arginine deiminase	10.2	−0.8	−0.8	7.3
SAOUHSC_01128, arcB1	Ornithine carbamoyltransferase	1.0	6.2	5.6	4.2
SAOUHSC_02968, arcB2	Ornithine carbamoyltransferase	10.1	−0.8	−0.7	7.3
SAOUHSC_01129, arcC1	Carbamate kinase	0.8	5.3	4.9	3.2
SAOUHSC_02965, arcC2	Carbamate kinase	8.5	−0.6	−0.7	5.8
SAOUHSC_02967, arcD	Arginine/ornithine antiporter	9.6	−0.7	−0.6	6.9
SAOUHSC_02964, argR	Transcriptional regulator	8.4	−0.6	−0.6	5.8
Peptide transport genes
SAOUHSC_01450 potE	Amino acid permease	1.8	5.7	6.1	5.2
SAOUHSC_02763	Peptide ABC transporter	−3	3.2	3.2	−0.1
SAOUHSC_02764	Peptide ABC transporter	−3.2	3.4	3.3	−0.1
SAOUHSC_02766	Peptide ABC transporter	−2.3	2.9	2.9	−0.1
SAOUHSC_02741	Amino acid ABC transporter permease	2.5	0.9	0.9	1.5
SAOUHSC_02767	Peptide ABC transporter	−3.0	3.7	3.9	0.0

^aSCV mutant AH875 (menB D151N).

^bGrowth-compensated SCV mutant AH1208 (menB D151N srrA M55I).

^cGrowth-compensated SCV mutant AH1131 (menB D151N srrB V420D).

^dSCV mutant AH862 (hemD Q107fs).