FIG 2 .

Identification of A. oris coaggregation-defective mutants by Tn5 transposon mutagenesis. Thirty-three A. oris coaggregation-defective Tn5 mutants identified by a cell-based screen were confirmed by a standard coaggregation assay (50). Equal cell numbers of A. oris mutants and S. oralis So34 were mixed together, and coaggregation was imaged using an AlphaImager. Coaggregation scores indicate the degrees of coaggregation, with the phenotype of the parental MG1 strain scored as 4 and that of the fimA, cafA, and srtC2 deletion mutants scored as 1; scores of 2 and 3 represent small and larger clumps of bacterial aggregates. Tn5 target genes were mapped by TAIL-PCR.