FIG 5 .

Requirement for NuoA and UbiE in oxidation of the thiol-disulfide oxidoreductase MdbA (A to D). Cells of the indicated A. oris strains were subjected to immunogold labeling with anti-FimA as described in the legend to Fig. 4. Scale bars indicate 0.5 µm. (E) Whole-cell lysates of A. oris strains were prepared by mechanical disruption and treated (+) or not treated (−) with Mal-PEG. Protein samples were immunoblotted with antibodies against the thiol-disulfide oxidoreductase MdbA (α-MdbA). A reduced form of MdbA is shown by an asterisk, whereas the MdbA species labeled by Mal-PEG at C169 are indicated by a black arrowhead.