TABLE 1 .

Mapping of A. oris coaggregation-defective Tn5 mutants

Tn5 mutant	Genomic Tn5 positiona	Target gene	Predicted function
1	2422225	cafA	Coaggregation factor A
2	38189	fimA	Type 2 fimbrial shaft pilin
3	36541	fimB	Type 2 fimbrial tip pilin
4	2420317	cafA	Coaggregation factor A
5	2421799	cafA	Coaggregation factor A
6	38174	fimA	Type 2 fimbrial shaft pilin
7	39115	srtC2	Type 2 pilus-specific sortase
8	39481	srtC2	Type 2 pilus-specific sortase
9	39749	srtC2	Type 2 pilus-specific sortase
10	2421087	cafA	Coaggregation factor A
11	2421136	cafA	Coaggregation factor A
12	38721	fimA	Type 2 fimbrial tip pilin
13	2420135	cafA	Coaggregation factor A
14	1755107	ana_1625	Geranylgeranyl reductase
15	1744569	nuoJ	NADH dehydrogenase I, subunit J
16	230679	ana_0218	Zn2+/Mn2+ transport system substrate-binding protein
17	1754944	nuoA	NADH dehydrogenase I, subunit A
18	162115	ana_0143	β-d-Glucoside glucohydrolase
19	1747764	nuoG	NADH dehydrogenase I, subunit G
20	1748226	nuoG	NADH dehydrogenase I, subunit G
21	1741097	nuoM	NADH dehydrogenase, subunit M
22	2348384	cstA	Carbon starvation protein A
23	2863369	ana_2659	Ni/Fe-hydrogenase III large subunit
24	2711713	ana_2514	Histidine kinase (2-component system)
25	2295587	ana_2118	Permease component of ABC-type multidrug transport system
26	1746526	nuoH	NADH dehydrogenase I, subunit H
27	382186	ana_0363	Conserved hypothetical protein
28	2566963	ana_2372	Hypothetical protein with Ser/Arg repeats
29	1745086	nuoI	NADH dehydrogenase I, subunit I
30	1739922	nuoM	NADH dehydrogenase I, subunit M
31	2513949	ana_2325; ana_2326b	Ana_2325 (Cys/His-dependent amidohydrolase/peptidase); Ana_2326 (hypothetical protein)
32	1712411	ana_1580	Conserved hypothetical protein
33	1080892	ana_1004	Hypothetical protein
34	2576712	ana_2380	Conserved hypothetical protein

^aShown is the nucleotide position of the Tn5 transposon mapped in the genome.

^bThe Tn5 insertion was found within the intergenic region of ana_2325 and ana_2326.