FIG 8 .

Timing and T2S dependency of ChiA translocation. U937 cells were infected for 6, 10, and 16 h with either WT 130b (A) or the WT carrying pMchiA (B) and were then labeled with LPS (upper-right boxes) and ChiA (lower-right boxes) antisera and analyzed by confocal microscopy. Host and bacterial DNAs were stained with DAPI, and the merged images appear as the larger boxes on the left side. (C) Quantification of percent ChiA localization (± SD) to the LCVM over time, based on the combined results from three independent trials. (D) U937 cells were infected with WT 130b and lspF mutant NU275, permeabilized with TX-100 or MeOH, and analyzed by confocal microscopy with DAPI and using ChiA and LPS antisera. The images presented show a portion of the cell containing the LCV and are representative of three independent experiments.