FIG 9 .

Galectin localization in L. pneumophila-infected macrophages. (A) U937 cells, PBMCs differentiated with human serum, BMD macrophages from A/J mice, and RAW 264.7 cells were infected with WT 130b for 16 h or were left uninfected (rightmost column) and were analyzed by confocal microscopy with galectin-3 (Santa Cruz Biotechnology) (left) and LPS (center) antisera. Host and bacterial DNAs were stained with DAPI, and merged images appear in the right column. (B and C) U937 cells were infected with WT 130b for 2, 4, 6, 8, 10, and 12 h (B) or with the lspF mutant NU275 for 16 h (C) and were then analyzed by confocal microscopy with galectin-3 (Santa Cruz Biotechnology) and LPS antisera. Host and bacterial DNAs were stained with DAPI, and merged images appear in the right columns with the percentages of galectin-positive LCVMs (± SD) in the lower-right-hand corner. The images presented are representative of three independent trials.