TABLE 4.
Apparent kinetic transport parameters for methamphetamine, amphetamine, and p-OHMA for hMATE1 and hMATE2-K from simultaneously modeling active and passive accumulation
Results represent Mean ± S.D. of three independent experiments each run in triplicate (Fig. 5; Supplemental Fig. 5).
| Transporter |
Methamphetamine |
Amphetamine |
p-OHMA |
|||
|---|---|---|---|---|---|---|
|
Km |
Vmax |
Km |
Vmax |
Km |
Vmax |
|
| μM | pmol/mg/min | μM | pmol/mg/min | μM | pmol/mg/min | |
| hMATE1 | 20.6 ± 4.5 | 86.6 ± 54 | 14.1 ± 4.9 | 238 ± 141 | 49.8 ± 26 | 257 ± 190 |
| hMATE2-K | 18.1 ± 11 | 97.7 ± 25.3 | 16.4 ± 12.2 | 92.9 ± 8.5 | NA | NA |
NA, not applicable.