Skip to main content
. 2005 Jan 21;102(5):1466–1471. doi: 10.1073/pnas.0409650102

Fig. 3.

Fig. 3.

A cell permeant fluorescent derivative of IP3RCYT blocks caspase activation and cell death in HeLa cells. (A) Schematic diagram of IP3RCYT peptide conjugated to BODIPY 577/618 via maleimide linkage to the C-terminal cysteine. (B) Phase and confocal fluorescent images of HeLa cells incubated with or without 400 nM BODIPY-IP3RCYT. Image capture times were equal. Note the diffuse cytoplasmic localization. (C) HeLa cells pretreated for 30 min with varying concentrations of BODIPY-IP3RCYT were stimulated with 1 μM STS for 6 h. Caspase activity was measured fluorometrically by using Z-DEVD-AMC as a substrate. Data were fit by using the Hill equation. Maximal inhibition was ≈30% of basal caspase activity (Inset). (D) HeLa cells pretreated with 400 nM BODIPY-IP3RCYT were stimulated with 1 μM STS for 6 h. Viability was assessed by trypan blue staining of nuclei. Cells were counted blind to the treatment, and at least 5,000 cells were scored over three separate experiments. *, P < 0.01.