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Fas-mediated cell death is attenuated by BODIPY-IP3RCYT. (A) Phase and confocal fluorescent images of Jurkat cells incubated with or without 400 nM BODIPY-IP3RCYT. Image capture times were equal. (B and C) Jurkat cells pretreated with 400 nM BODIPY-IP3RCYT or vehicle for 30 min and 1 ng/ml FasL vesicles were added for the indicated amount of time. Trypan blue staining (B) and DEVD-cleavage activity (C) were assayed as in Fig. 3. *, P < 0.05.
