FIG 4.

Kinetics of cell subsets and cytokine-producing cells in the lungs of Pneumocystis-infected CD40L-KO mice. The percentage of CD3⁺ T cells and γδ T cells as a percentage of total lymphocytes and CD4⁺ T cells as a percentage of CD3 cells (top row) and the percentage of CD3⁺ T cells that produce either IFN-γ or IL-17 (bottom row left and middle panels) that were isolated from lungs of Pneumocystis-infected mice over time in one experiment were determined. The Pneumocystis burden from the same animals is shown in the bottom right panel. The results represent the means and standard deviations of mice from all cages within a single experiment at a given time point. The time points studied (days 36 to 45 after start of cohousing with a seeder) are those during which responses in C57BL/6 mice were maximal. Due to the limitations in the number of animals that could be housed per cage, two exposed animals were studied at each time point per experiment, except as indicated by the absence of error bars, when only one animal was available for analysis. Three controls were included in the study. There were no significant differences in any of the cell populations compared to uninfected controls at any time point.