FIG 6.

TLR2 deficiency leads to impaired Mincle upregulation on the cell surface. Shown are data from flow cytometric analysis of Mincle receptor expression on TLR2-deficient BMM and the respective wild-type controls. BMM were stimulated with 30 μg/ml cell wall extract of C. diphtheriae ISS4749 or C. ulcerans for 48 h. Unstimulated cells (mock) or BMM stimulated with 10 ng/ml LPS or 50 ng/ml Pam3CSK4 were used as controls. Panel A depicts representative histograms, indicating the gate used for the determination of Mincle-expressing macrophages shown in panel B. Data are depicted as single values from 2 independent experiments with biological duplicates (n = 4).