FIG 5.

Anesthetized animals display significantly more CD11b^hi Ly-6G⁺ NOS2⁺ cells in the kidney during MRSA bloodstream infection. (A) Animals were infected with 3 × 10⁶ CFU of S. aureus via tail vein. Animals were sacrificed at 14 days (top) or 32 days (bottom) postinfection. Kidneys were isolated and processed for FACS analysis. (Right) At both 14 and 32 days postinfection, propofol-treated animals contained notably large numbers of CD11b^hi Ly-6G⁺ NOS2⁺ cells that resembled myeloid-derived suppressor cells (MDSCs) (cell populations in purple). (Left) This cell population was largely absent from the kidneys of control mice. (B) Propofol-treated mice contained more MDSC-like cells at 14 days (in purple; left) and 32 days (right) postinfection. Data are representative of 2 independent experiments with 5 animals/treatment group/time point. **, P < 0.01.