Skip to main content
. 2017 Jun 20;85(7):e00218-17. doi: 10.1128/IAI.00218-17

FIG 4.

FIG 4

Drosophila PGRP-LC7454 and RelE20 mutants are more susceptible to C. burnetii NMII clone 4. (A to F) Adult w1118, PGRP-LC7454, and RelE20 male flies, 4 days of age, were mock infected or infected with C. burnetii (100 GE/fly). Percent survival was evaluated for a period of 20 days, comparing infected flies to one another (A) or mock- and Coxiella-infected flies for each genotype (B to D). (E) Bacterial loads were determined at 6 and 20 days postinfection by qPCR. (F) Expression of Drosocin in w1118, PGRP-LC7454, and RelE20 flies was determined at 12 days postinfection by reverse transcriptase quantitative real-time PCR, and the results were normalized to the Drosophila RpII transcripts. (G to I) Four-day-old sibling adult flies carrying a UAS-induced dsRNA cassette targeting Relish (TRiP.HMS00070) or PGRP-LC (TRiP.HMS00259) with an Actin5C-driven GAL4 element (GAL4 > UAS) or lacking the GAL4 element (+ > UAS) were infected with C. burnetii (100 GE/fly). (G) Percent survival was evaluated for a period of 30 days. (H) The bacterial loads were determined at 6, 20, and 30 dpi by qPCR. (I) Expression of Drosocin was determined at 12 dpi. The asterisks denote statistical significance (*, P < 0.05). The error bars indicate standard deviations.