Skip to main content
. 2017 Jun 16;83(13):e00672-17. doi: 10.1128/AEM.00672-17

FIG 2.

FIG 2

Mutations in HRR25 increase the expression of the TPO2-lacZ and YRO2-lacZ reporter genes. (A) Generation of hrr25(E52D) missense mutant strains via tetrad analysis. BY4743 hrr25Δ::kanMX4/HRR25 diploid cells containing a centromeric plasmid carrying an hrr25(E52D) mutant allele (pZD359) were sporulated, and tetrads were dissected on a YPD plate. The picture was taken after 3 days. M, hrr25(E52D) mutants; W, wild type. (B) Generation of hrr25Δ mutant strains via tetrad analysis of BY4743 hrr25Δ::kanMX4/HRR25 cells. Small colonies were hrr25Δ mutants. The picture was taken after 6 days. (C and D) The effect of hrr25(E52D) and hrr25Δ mutations on TPO2-lacZ and YRO2-lacZ reporter genes. Wild-type (BY4741) and isogenic hrr25(E52D) (ZLY4467) and hrr25Δ (ZLY4501) mutant cells were grown in MM4 without acetic acid (C) and with 60 mM acetic acid (D). β-Galactosidase activity assays were carried out as described in Materials and Methods.