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Yeast two-hybrid analysis of an interaction between Hrr25 and Haa1. AH109 cells expressing GBD, GAD, GBD-HRR25, GBD-HRR25(KD), or GAD-HAA1 as indicated were streaked on SD medium with histidine and adenine and CSM dropout medium without histidine and with or without 3 mM 3-AT. Pictures were taken after 4 days at 30°C.
