FIG 1.

Experimental setup and evolutionary trajectories. (A) “Static ALE” exposes cultures to a constant environment, while a “dynamic ALE” introduces temporal variability in carbon growth substrate. (B) Example plot of fitness trajectories (i.e., growth rates) for statically evolved (pink/purple) and switching (dark green/light green) cultures on glucose and xylose over the course of the ALE experiment. Similar fitnesses are reached, although under switching conditions cultures take longer to get there. CCD; cumulative cell divisions; Glc, glucose; Xyl, xylose; Gly, glycerol; Ac, acetate.