FIG 5.

AHPND-causing V. parahaemolyticus strains contain a functional antibacterial T6SS1. (A) Expression (Cell) and secretion (Media) of T6SS1 component VgrG1 by the indicated V. parahaemolyticus strains grown in MLB at 30°C ± 20 μM phenamil were analyzed by Western blotting using α-VgrG1 antibody. PAR, parental strain. LC, loading control. (B and C) Viability counts of E. coli (B) or V. cholerae (C) prey before (0 h) and after (4 h) coculture with indicated V. parahaemolyticus attacker strains on MLB (3% NaCl) solid media at 30°C. Data are representative of three independent experiments.