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β-Lactam antibiotic resistance assaya
| E. coli strain | MIC (μg/ml) |
|||||
|---|---|---|---|---|---|---|
| PENG | AMP | AMO | CAR | CEF | CEFD | |
| Origami 2(DE3)/pET-28b | 16 | 8 | 8 | 8 | <8 | 8 |
| Origami 2(DE3)/pMQ28 | 64 | 16 | 64 | 16 | 128 | 128 |
PENG, penicillin G; AMP, ampicillin; AMO, amoxicillin; CAR, carbenicillin; CEF, cephalexin; CEFD cefadroxil. The tested concentrations of antibiotics were 8, 16, 32, 64, 128, 256, and 512 μg/ml. The plasmid pMQ28 contains the cloned macQ gene, and the plasmid pET-28b is an empty vector control.
