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. 2017 Jun 16;83(13):e00337-17. doi: 10.1128/AEM.00337-17

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FIG 2 — Phylogenetic BioNJ tree obtained for relaxases (OrfJ) of ICEs belonging to the ICESt3 subfamily. The relaxase protein sequence of 27 ICEs (the 19 ICEs of the ICESt3 subfamily with closely related conjugation modules and 8 additional ones previously reported to belong to the same ICE subfamily but showing more distantly related conjugation modules) were included in the analysis. ICEs of S. salivarius are indicated in bold, and ICE_Sma33MO_fda is indicated by an asterisk. Bootstrap values supporting main branches are given for BioNJ and ML, respectively.