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PCR detection of integrated and excised forms of ICE_SsaF1-4_fda. The sizes of the PCR fragments obtained for the amplification of the attB, attI, attR, and attL sites (586 bp, 887 bp, 900 bp, and 523 bp, respectively) were confirmed by parallel migration of a DNA ladder. The primer pairs used for these amplifications are listed in Table 2.
