FIG 5.

Characterization of transconjugants carrying ICE_SsaF1-4_fda after intraspecies transfer. F1-4(ICE_SsaF1-4_fda) was used as the donor in mating experiments with JIM8777(pMG36e) (a) and F1-8(pMG36e) (b and c) as recipients. Tc, transconjugants; integrase, amplification of the integrase gene of ICE_SsaF1-4_fda; pMG36e, amplification of an internal fragment of the pMG36e plasmid; RAPD, random amplification of polymorphic DNA; attL, attR, attI, and attB, amplification of fragments carrying these attachments sites in F1-8 transconjugants. The primer pairs used for these amplifications are listed in Table 2.