FIG 6.

Characterization of transconjugants carrying ICE_SsaF1-4_fda after interspecies transfer. F1-4(ICE_SsaF1-4_fda) was used as the donor in mating experiments with LMG18311(pMG36e) (a) and JH2-2(pMG36e) (b) as recipients. Tc, transconjugants; integrase, amplification of the integrase gene of ICE_SsaF1-4_fda; ITS16S/23S, amplification of the internal transcribed spacer; ITS16S/23S+HaeIII digestion, amplification of the internal transcribed spacer followed by HaeIII digestion of the obtained fragment; EF fda, amplification of fda fragment using E. faecalis-specific primers.