TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or genotype | Source or reference |
|---|---|---|
| Strains | ||
| S. salivarius | ||
| B35 | WTa strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| B57 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| F1-4 | WT strain carrying a putative ICE of the ICESt3 subfamily (ICE_SsaF1-4_fda) | 23 |
| F1-4(ICE_SsaF1-4_fda) Cmr | F1-4 carrying ICE_SsaF1-4_fda tagged with a Cmr cassette | This work |
| F1-8 | WT strain carrying a putative ICE in accretion with an IME integrated in the 3′ end of rpmG; no element in fda | 23 |
| F1-8(pMG36e) | F1-8 carrying pMG36e, a plasmid conferring erythromycin resistance | This work |
| F4-2 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| F6-1 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| JIM8777 | WT strain carrying a putative IME in the 3′ end of rpmG and a putative CIME in the 3′ end of rpsI; no element integrated in the fda gene | 57 |
| JIM8777(pMG36e) | JIM8777 carrying pMG36e, a plasmid conferring erythromycin resistance | This work |
| L22 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| L50 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| L60 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| L64 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| N5 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| N20 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| T93 | WT strain carrying a putative ICE of the ICESt3 subfamily | 23 |
| S. thermophilus | ||
| CNRZ385(ICESt3cat) | CNRZ385 carrying ICESt3 with cat inserted in orf385J pseudogene; Cmr | 24 |
| LMG18311 | WT strain with no element in fda | BCCM/LMG |
| LMG18311(pMG36e) | LMG18311 carrying pMG36e, a plasmid conferring erythromycin resistance | 24 |
| E. faecalis | ||
| JH2-2 | WT strain with no element in fda | 58 |
| JH2-2(pMG36e) | JH2-2 carrying the plasmid pMG36e, conferring erythromycin resistance | 24 |
| Plasmids | ||
| pSET5s | pWV01-type thermosensitive replication origin from pVE6002; LacZ Cmr | 59 |
| pMG36e | 3.6 kb, replication origin from pWV01; Eryr | 49 |
a
WT, wild type.