FIG 3.

Low-resolution homology model of EfAmy. EfAmy residues that were chosen for mutation to Pro are indicated in black. (A) The catalytic dyad (Glu234 and Asp210) and the other residues involved in the interaction with the ligand ABC (reporting the accepted nomenclature from +3 to −3; see Materials and Methods and reference80). Feasible nonpolar interactions indicated with dotted lines and their lengths in Å are also reported. (B) The same region shown in panel A with V212T and V232T mutations. The most likely formed hydrogen bonds are indicated by dotted lines with their lengths in Å.