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(A to C) Effect of temperature on the amylolytic activity of EfAmy, EcAmy, and mutants. For each enzyme, the total activity at the optimal temperature was set at 100%. This represented 1.27 U/ml for EfAmy, 1.86 U/ml for EcAmy, 1.31 U/ml for the E166P mutant, 1.34 U/ml for S185P, 1.31 U/ml for T350P, 1.53 U/ml for V212T, 1.51 U/ml for V232T, 1.91 U/ml for V212T/V232T, 1.32 U/ml for E166P/S185P/T350P, and 2.03 U/ml for E166P/S185P/T350P/V212T/V232T.
