TABLE 1.
Transfer of pC3 allows mingling of metabolic phenotypes between strainsa
| Substrateb | H111 | H111Δc3 | H111/pC3K56-2 | K56-2 | K56-2Δc3 | H111/pC3383 | 383 | 383Δc3 | Apparent source of phenotype |
|---|---|---|---|---|---|---|---|---|---|
| N-Acetyl d-glucosamine | +++ | +++ | +++ | − | − | +++ | − | − | H111 c1 or c2 |
| Quinic acid | +++ | +++ | +++ | − | − | +++ | − | − | H111 c1 or c2 |
| Tricarballylic acid | − | − | +++ | +++ | − | − | − | − | K56-2 pC3 |
| m-Tartaric acid | − | − | − | − | − | +++ | +++ | − | 383 pC3 |
a
Phenotypic microarray analysis was used to analyze the indicated strains. In each case, the hybrid strain was compared with the wt parent and pC3 donor strains. +++, a ≥4-fold increase in OD590; −, the OD590 was equal to or lower than that of the negative control.
b
Substrates shown gave rise to a difference in final OD590 of >4×.