TABLE 2.
Comparison between morphological identification and NGS results for pollena
| Taxonomic level: order | Pollen type | A1 |
B1 |
C1 |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Morphology |
NGS (% DNA sequences) | Morphology |
NGS (% DNA sequences) | Morphology |
NGS (% DNA sequences) | |||||
| No. of pollen grains/m3/wk | % total grains | No. of pollen grains/m3/wk | % total grains | No. of pollen grains/m3/wk | % total grains | |||||
| Apiales | Apiaceae | 0 | 0.00 | 0.05 | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.01 |
| Asterales | Artemisia | 1 | 0.33 | 0.06 | 2 | 0.67 | 0.20 | 2 | 0.36 | 0.4 |
| Compositae (others) | 2 | 0.66 | 0.24 | 1 | 0.34 | 0.10 | 1 | 0.18 | 0.1 | |
| Caryophyliales | Caryophyllaceae | 0 | 0.00 | 0.20 | 0 | 0.00 | 0.09 | 0 | 0.00 | 0.01 |
| Chenopodiaceae/Amaranthaceae | 0 | 0.00 | 0.01 | 4 | 1.35 | 0.01 | 3 | 0.54 | 0.00 | |
| Ericales | Ericaceae | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.00 |
| Fabales | Sophora/Styphnolobium | 0 | 0.00 | 16.00 | 0 | 0.00 | 22.00 | 0 | 0.00 | 14.00 |
| Fabaceae (others) | 2 | 0.66 | 2.00 | 2 | 0.67 | 1.00 | 3 | 0.54 | 1.00 | |
| Fagales | Alnus | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.06 | 1 | 0.18 | 0.04 |
| Corylus | 0 | 0.00 | 0.01 | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | |
| Quercus | 3 | 0.99 | 0.30 | 8 | 2.69 | 0.40 | 12 | 2.14 | 2.00 | |
| Lamiales | Fraxinus | 2 | 0.66 | 0.50 | 3 | 1.01 | 0.10 | 9 | 1.61 | 1.00 |
| Ligustrum | 0 | 0.00 | 0.07 | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.03 | |
| Olea | 0 | 0.00 | 0.00 | 3 | 1.01 | 0.00 | 0 | 0.00 | 0.00 | |
| Plantago | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.05 | 0 | 0.00 | 0.00 | |
| Malpighiales | Populus | 0 | 0.00 | 3.00 | 1 | 0.34 | 2.00 | 0 | 0.00 | 2.00 |
| Myrtales | Eucalyptus | 1 | 0.33 | 0.00 | 0 | 0.00 | 0.00 | 0 | 0.00 | 0.00 |
| Pinales | Cupressaceae/Taxaceae | 272 | 89.77 | 37.00 | 243 | 81.82 | 52.00 | 501 | 89.46 | 60.00 |
| Pinaceae | 1 | 0.33 | 0.00 | 4 | 1.35 | 0.00 | 4 | 0.71 | 0.00 | |
| Poales | Poaceae | 5 | 1.65 | 6.00 | 9 | 3.03 | 5.00 | 6 | 1.07 | 3.00 |
| Proteales | Platanus | 2 | 0.66 | 1.00 | 0 | 0.00 | 0.40 | 0 | 0.00 | 0.60 |
| Rosales | Celtis | 0 | 0.00 | 0.50 | 0 | 0.00 | 0.20 | 0 | 0.00 | 0.20 |
| Ulmus | 0 | 0.00 | 13.00 | 0 | 0.00 | 11.00 | 0 | 0.00 | 9.00 | |
| Urticaceae | 7 | 2.31 | 16.00 | 13 | 4.38 | 4.00 | 16 | 2.86 | 5.00 | |
| Sapindales | Acer | 0 | 0.00 | 0.20 | 0 | 0.00 | 0.05 | 0 | 0.00 | 0.01 |
| Unidentified | 5 | 1.65 | 1.91 | 4 | 1.35 | 0.44 | 2 | 0.36 | 0.79 | |
| Others (only NGS) | 0 | 0.00 | 1.95 | 0 | 0.00 | 0.90 | 0 | 0.00 | 0.80 | |
| Total | 303 | 100.00 | 100.00 | 297 | 100.00 | 100.00 | 560 | 100.00 | 100.00 | |
a
Pollen types with no representatives in either of the two analyses have been omitted.