FIG 3.

Prolonged intra-amoebal survival of Y. pestis KIM strains in the absence of gentamicin. Intracellular survival of Y. pestis excluding gentamicin maintenance of cocultures at 25°C. Error bars represent the mean ± SD of the results from 3 to 6 independent experiments. For assays with KIM5 (blue), KIM6⁺ (orange), and the KIM5 T3SS mutant (yellow), horizontal bar indicates comparison between two time points at an MOI of 100, while vertical bar indicates comparisons between MOI of 1 (triangles) and 100 (circles). ns means not statistically significant.