TABLE 1.
Twenty-three transposon insertion mutants of B. cereus AR156
| Strain | Insertion site (AR156) (nt) | Gene locus (ATCC 14579) | Description (ATCC 14579)a |
|---|---|---|---|
| BC41 | 1283251 | BC_5055 | Possible wall-associated protein/M60 family peptidase |
| BC42 | 132817 | BC_3827 | ATP-dependent hsl protease ATP-binding subunit HslU (clpY) |
| BC44 | 3887313 | BC_2307 | Bacillibactin synthetase component F/adenylation domain of nonribosomal polyketide synthase |
| BC50 | 473010 | BC_4190 | Stage III sporulation protein AD (spoIIIAD) |
| BC52 | 591920 | BC_4325 | Putative pyrroline-5-carboxylate reductase (comER) |
| BC56 | 4475863 | Intergenic region between BC_4741 and BC_4742 | Downstream of TrkA domain site-specific recombinase (BC4171), upstream of ABC transporter NapA (BC4172) |
| BC58 | 1089845 | BC_4842 | Transcriptional regulator, GntR family |
| BC61 | 2003883 | BC_0334 | Phosphoribosylamine-glycine ligase (purD) |
| BC65 | 2386938 | BC_2456 | NRPS |
| BC68 | 1667177 | BC_5481 | Stage 0 sporulation protein J (soj) |
| BC79 | 1122960 | BC_4867 | 1,4-Alpha-glucan branching enzyme (glgB) |
| BC83 | 789947 | BC_4540 | DUF2196 superfamily (ywbE, in the putative operon of holin-antiholin genes cidAB in B. subtilis) |
| BC85 | 2002949 | BC_0333 | Phosphoribosyl-aminoimidazole carboxamide formyltransferase/IMP cyclohydrolase (purH) |
| BC98 | 626575 | BC_4365 | Aldehyde-alcohol dehydrogenase (aad) |
| BC102 | 1003735 | BC_4755 | PAP2 family protein, membrane-associated phospholipid phosphatase |
| BC105 | 2059639 | BC_0382 | Ferric-chrome ABC transporter (fluB) |
| BC108 | 1875050 | BC_0221 | Molybdenum ABC transporter (modA) |
| BC110 | 183948 | BC_3875 | Xaa-Pro dipeptidase (pepP) |
| BC125 | 2374525 | BC_0685 | Branched-chain amino acid transport system II carrier protein (brnQ) |
| BC133 | 1298318 | BC_5070 | Small function-unknown gene (BC_5070), downstream of multidrug efflux protein (BC5071) |
| BC143 | 1475960 | Intergenic region between BC_5292 and BC_5291 | Immediately downstream of NADH-ubiquinone dehydrogenase, M subunit (BC_5292), and upstream of N subunit (BC_5291) |
| BC150 | 2081676 | BC_0407 | OTCace |
| BC157 | 1817740 | BC_0167 | N-Acetylmuramoyl–l-alanine amidase (cwlD) |
a
NRPS, nonribosomal peptide synthetase; PAP2, type 2 phosphatidic acid phosphatase; OTCace, ornithine carbamoyltransferase.