FIG 3.

Transcription activity of the hcnC gene in wild-type P. brassicacearum LBUM300 and strain LBUM300ΔphlD in the rhizosphere of tomato plants detected at 24 h, 72 h, 120 h, 168 h, 216 h, and 264 h postinoculation using RT-qPCR (n = 8 for each treatment). hcnC gene transcripts were normalized to phlD gene copy number per gram of rhizosphere soil. Strain LBUM300ΔhcnC, carrying a disrupted hcnC gene and incapable of hcnC expression and HCN production, is represented by an asterisk. The mean of all times within each treatment is indicated by a white bar, and treatments showing different letters are significantly different (P < 0.05). Error bars are standard errors of the means.