FIG 2.

Schematic structures and genus-wide screening of the putative AbFs in Caldicellulosiruptor species. (a) Schematic diagram of two GH3s, five GH43s, one AbF51, a multimodular XynF enzyme, and two truncated mutants of XynF. (b) SDS-PAGE analysis of two GH3s, five GH43s, and one AbF51 from Caldicellulosiruptor sp. strain F32. (c) Heterologous recombination of a full-length XynF in E. coli by high-resolution mass spectroscopy-guided mutagenesis. (d) Specific activities of AbF51 and XynF on the different substrates.