FIG 3.

Biochemical characterizations of XynF and AbF51. (a) Effect of pH and temperature on the activities of XynF. For optimum pH conditions, citrate buffer was used at pH 4 to 6 (black line) and phosphate buffer at pH 6 to 8 (red line). (b) Thermostability of XynF at different temperatures. (c) Effect of pH and temperature on the activities of AbF51. (d) Thermostability of AbF51 at different temperatures. Wheat arabinoxylan and pNP-AraF were used as substrates for XynF and AbF51, respectively. The kinetic parameters of XynF and AbF51 were examined under optimum pH and temperature conditions.