FIG 7.

Hydrolysis actions of two AbFs on arabinose-branched XOSs. (a and b) WAX (1%) was hydrolyzed by XynA for 12 h at 70°C. The products were examined by HPAEC-PAD (a) and ¹H-NMR (b). (c and d) WAX (1%) was hydrolyzed by XynA for 12 h followed by AbF51 for 12 h at 70°C. The products were examined by HPAEC-PAD (c) and ¹H-NMR (d). (e and f) WAX (1%) was hydrolyzed by XynA for 12 h followed by XynF for 12 h at 70°C on HPAEC-PAD (e) and ¹H-NMR (f). A, X1, X2, X3, and X4 refer to arabinose, xylose, xylobiose, xylotriose, and xylotetrose, respectively. The signals of 2-O-linked (δ 5.28) and 3-O-linked (δ 5.33) arabinoses were assigned.