Table 4.

Correlation between age and DNA integrity; mean and standard deviation of percentages of sperm cells with chromatin alterations detected by the CMA3, 8-OhdG and SCSA techniques of young (n = 27), adult (n = 59), and aged (n = 36) bovine semen samples

Group	CMA3	8-OHdG	SCSA (%DFI)
Young	1.57 ± 0.76ª	25.69 ± 6.29c	3.55 ± 0.61ª
Adult	1.09 ± 0.63b	34.89 ± 9.20b	2.13 ± 0.78c
Aged	0.90 ± 0.59b	44.11 ± 11.00a	3.09 ± 0.96b
Correlation (P value)	−0.33 (>0.0001)	0.63 (>0.0001)	−0.14 (0.1401)

Means with different superscripts in the same column differ significantly (p < 0.05) by the Duncan test