Figure 2. Effect of stable overexpression of VEGF on macrophages in tubular networks formation.

After 24 h of culture in Matrigel, hVEGF₁₆₅-ZsGreen1-RAW264.7 formed tubular structures. But no similar structure was detected in untransfected RAW264.7, ZsGreen1-RAW264.7, or exogenous VEGF-treated RAW264.7. Scale bars =100 μm. All images are representatives of three independent experiments.