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. 2017 Jun 21;37(3):BSR20160478. doi: 10.1042/BSR20160478

Figure 1. Down-regulated FBXW7 and IGF1R may induced by overexpression of miR-223 in HCC827/ER cells.

Figure 1

Overexpression of miR-223 in HCC827/ER cells. (A) The levels of miR-223 in HCC827 cells during their treatment with serially diluted erlotinib (0, 0.1, 0.5, 1, and 2 µM) for 48 h as analyzed by q-PCR. (B) The levels of miR-223 in HCC827 and HCC827/ER cells as analyzed by q-PCR. (C and D) The binding sites and target genes (IGF1R and FBXW7) for miR-223 as predicted by Targetscan. Wild-type (FBXW7/IGF1R 3’-UTR-WT) or mutant (FBXW7/IGF1R 3′-UTR-MUT) reporter plasmids were co-transfected along with miR-223 or an NC into 293T cells. Normalized luciferase activity in the control group was used for calculating relative luciferase activity. (E) IGF1R and FBXW7 mRNA levels in HCC827 and HCC827/ER cells as analyzed by q-PCR; *P<0.05 and ***P<0.001.