Fig. 2.

In situ analysis of PTPRG expression. a Immunohistochemistry using monoclonal TPγ B9-2 and chPTPRG antibodies on cryostatic sections of mouse testis. In the heterozygous PTPRG (+/−) mice, shown in the insets, the two antibodies recognize the same structures while in Ptprg-null (KO) (−/−) mice, shown as the main figure, no staining was detectable. b Comparison between TPγ B9-2, chPTPRG, and RbtP4 in normal human lung (a–c) and pancreas (d–f). Formalin-fixed, paraffin-embedded sections were stained with anti-PTPRG antibodies indicated. All the antibodies recognize lung alveolar macrophages (a–c, arrows). In pancreas (d–f) TPγ B9-2 preferentially stains Islets of Langerhans (white arrows) and centroacinar regions (black arrows), while chPTPRG stains the tissue more diffusely, including the exocrine glands. Matched irrelevant antibodies controls (murine IgG₁, IgY, and rabbit IgG) are shown in the insets